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LABORATORY PROCEDURES FOR MICROORGANISMS

Appendix

M/1998/2.05 Appendix 1


Selected tests used in the characterisation and quality control of bacteria

Example

 Morphological and staining characteristics

Shape

Arrangement

Irregular forms

Sheaths

Budds

Appendages

Prosthecae

Stalks

Holdfasts

Motility, swimming

Motility, gliding

Gram reaction

Acid fastness

Cell inclusions

Flagella, number and insertion

Size

Ends

Change during growth phase

 

Cultural characteristics

Colonies

Shape opacity

Size consistency

elevation emulsifiability

edge surface

Growth in broth

Surface

Turbidity

deposit

Physiological characteristics

Phototrophic

Chemoautotrophic

Chemoheterotrophic

Aerobic growth

Anaerobic growth

Phage susceptibility

Temperature range

Optimum temperature

vpH range

Nutrition

Growth factors

Serology

Gaseous needs

Resistance to antibiotics

Growth on selective media

Growth in presence of NaCl

Heat resistance

 

Biochemical characteristics

Metabolic pattern

Specific enzyme tests

Commercial identification kits

Oxidative/fermentative

utilisation of sugars

Utilisation of substrates

Production of specific metabolites

 

Chemotaxonomical characteristics

Fatty acids, lipids

Cell wall composition

Ubi-/Menaquinones

Polyamines

 

Protein pattern

Hopanoids

 

DNA techniques

Mol% G+C

DNA hybridization

RAPD

AFLP

RFLP

Sequencing

 


Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
Page layout by CERDIC
Copyright CABRI, 1998

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Site maintained by Paolo Romano. Last revised on February 2023.